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Legionella Detection Test Kit | Rapid Test Method

Accepta’s innovative rapid Legionella detection test kit uses the latest "quick-test" technology to allow the rapid in-field detection of Legionella pneumophila sero group 1.

Accepta's rapid Legionella tests are multi-use analysis kits designed for all types of waters including cooling towers, domestic hot and cold water systems, showers, water features and many more applications where Legionella bacteria presents a risk safety and health.

legionella-detection-test-kit-rapid-testKey Benefits

  • Improves Legionella risk management procedures
  • Rapid detection of Legionella bacteria
  • Results achieved in 30-35 minutes
  • Easy to follow test procedure
  • Improves Legionella risk management procedures
  • Ideal for water treatment engineers
  • Compact and portable design
  • Detects Legionella pneumophila sero group 1 bacteria
  • Everything included to perform 5 tests

Accepta's rapid Legionella detection test kit uses an immunochromatographic assay to detect within 30 minutes the presence of cell surface antigens from Legionella pneumophila serogroup 1.

The presence of the antigen in the water causes the “Test Line” to turn red in colour. A “Control Line” is included which should always turn red if the test has been performed correctly.

The kit includes 5 tests and has been designed to test waters to a sensitivity of 100 CFU/L. It is ideal when access to laboratories is impractical or speed is imperative.

It should be used to supplement your existing sampling programme and should not replace routine laboratory analysis.


Bacteria detected

Legionella pneumophilaserogroup 1

Detection method

Lateral flow immunochromatographic assay

Lower limit of detection

Up to 100 CFU/L (0.1 CFU/mL)


Tested against a wide range of waterborne bacteria, including Acinetobactercalcoaceticus, Aeromonashydrophila subsp. hydrophila, Bacillus subtilis, Burkholderiacepacia, Citrobacterfreudii, Citrobacterkoseri, Escherichia coli, Enterobacter cloacae, Klebsiellaoxytoca, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Ralstoniapickettii, Raoultellaterrigena, Streptococcus pyrogenes, Yersinia ruckeri. No cross reactions observed at concentrations > 109 CFU/L.

Staphylococcus aureus has been observed to cross react with the test at concentrations > 108 CFU/L.

Legionella pneumophila sero groups 4 & 7 cross react with the test at concentrations >109 CFU/sample. The test does not react with any other L. pneumophila serogroups or L. species tested.

Test volume

100 μL

Suggested sample volume

250 mL

Sample parameters

Sample should filter in less than 10 minutes.


The product has been tested with a range of common biocides and   iodispersants used in Legionella control, at their normal maximum perating concentration. Active ingredients tested include:   lutaraldehyde, didecyl-dimethyl-ammonium chloride, isolthazoles, dibromonitrilopropionamide, ionic and non-ionic surfactants.  The test gives a false positive if used with polymeric biguanide or THPS.

Operator time

10 - 30 minutes

Development time

25 minutes

Shelf life

18 months from manufacture when stored at room temperature (<30°C) in original packaging.

The complete kit includes:

  • Plastic case with instructions
  • 1 x Universal Filtration Accessory Pack
  • 1 x 250 ml Sample Bottle
  • 5 x RLeg60070 - Legionella Test Cell
  • 5 x RLeg60080 - Legionella Filter
  • 5 x RLeg660090 - Legionella Syringe
  • 5 x RLeg60100 - Legionella Filtration Tube

Accepta order codes

  • RLeg60050F - Universal Test Kit inc filtration
  • RLeg60050 - Universal Rapid Legionella Test Kit
  • RLeg60060 - Universal Filtration Accessory Pack
  • RLeg60070 - Legionella Test Cell
  • RLeg60080 - Legionella Filter
  • RLeg60090 - Legionella Syringe
  • RLeg60100 - Legionella Filtration Tube

First time users should purchase RLeg60050F.

For further tests purchase RLeg60050 or single components.

Technical Support

If you require additional information or specialist technical support concerning our rapid Legionella test kit please contact Accepta's technical support team.

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